No in most style guides — "at" is lowercase in AP Style, Chicago Style, and MLA Style.
"At" is a two-letter preposition that is lowercased by most major style guides in the middle of a title. AP Style, Chicago Style, MLA Style, and AMA Style all lowercase it, while NYT Style and Billboard Style capitalize it.
The Chicago Style threshold of four letters places "at" comfortably in the lowercase category. "At" has only two letters, which is well below the point at which Chicago Style begins capitalizing prepositions.
When "at" appears as the first word of a title — less common but legitimate — it is always capitalized. The same applies to its rare use as a title's last word.
In informal writing and digital contexts, "at" also refers to the @ symbol. When used in a title as a preposition in its traditional sense, the standard title case rules apply.
"At" is one of the most common English prepositions and appears frequently in titles. Its treatment in title case is consistent with other short prepositions: lowercase in AP Style, Chicago Style, MLA Style, and AMA Style, and capitalized in NYT Style.